Responsible Research and Innovation – A Manchester Perspective.

What is Responsible Research and Innovation? Barbara Ribeiro and Philip Shapira have written a short briefing paper that provides an overview of responsible research and innovation (RRI), with concise highlights about principles of responsibility, the emergence of RRI in the UK and Europe, the EPSRC AREA framework, and RRI in Manchester. To read, go to: http://synbiochem.co.uk/rri-manchester-perspective/

An automated Design-Build-Test-Learn pipeline

The SYNBIOCHEM automated pipeline for the discovery and optimisation of biosynthetic pathways for microbial production of fine chemicals has been published in Nature: Communications Biology.

The microbial production of fine and speciality chemicals promises to deliver bio-sustainable manufacturing solutions of the future. In this article we present an integrated Design-Build-Test-Learn pipeline which is designed to be agnostic to target compound and applicable to any chemical compound of interest. The paper demonstrates the application of the pipeline for 2 of our core chemical classes with improved production titres.

Article can be found: https://www.nature.com/articles/s42003-018-0076-9

Faster genome evolution methods to transform yeast

Scientists have created a new way of speeding up the genome evolution of baker’s yeast Saccharomyces cerevisiae, the same yeast we use for bread and beer production.

This is to develop a synthetic yeast strain that can be transformed on demand, making it particularly attractive for industrial biotechnology applications, such as the mass production of advanced medicines to treat illnesses such as Malaria and Tuberculosis (TB). It could also have massive implications for the future study of DNA.

Led by Professor Patrick Cai at the Manchester Institute of Biotechnology, in collaboration with Prof. Junbiao Dai from the Shenzhen Institute of Advanced Technology at the Chinese Academy of Sciences, two back-to-back papers are being published in Nature Communications on May 22nd, 2018.

Read more

eForFuel: Fuels from CO2 and Electricity

C3 Biotechnologies Ltd is taking part in eForFuel, an exciting EU-funded research and innovation initiative that seeks to provide a sustainable replacement of fossil fuels by using electricity and microorganisms to convert CO2 into renewable fuels.

Read more

Updates can be found at www.eforfuel.eu and on Twitter @eforfuel

New – The University of Manchester Presidential Fellowships

The University is inviting the brightest academic talent to apply for The University of Manchester Presidential Fellowships. We’re interested in early-career academics who can deliver world-leading research and teaching, and become the inspiring leaders of the future. Applicants should have a high academic standing, a growing reputation in research, and the specialist knowledge needSYNBIOCHEM - About - Manchester Institute of Biotechnologyed to develop exemplary research programmes and methodologies. Applicants with an interest in SynBio/IB particularly welcome! See more: https://www.brightest-minds.manchester.ac.uk 

 

Selenzyme: Enzyme selection tool for pathway design

SYNBIOCHEM - Research

Enzymes are proteins found in every living organism, catalysing biochemical reactions and involved in multiple biological processes. Industrial biotechnology utilises enzymes in a large variety of applications such as in sectors including food, cosmetics, pharmaceuticals and advanced materials. However, finding the right combination of enzymes for a desired process is often extremely challenging. Selenzyme, developed by researchers at the Manchester Synthetic Biology Research Centre (SYNBIOCHEM), Manchester Institute of Biotechnology, is a freely available online tool that assists the scientist in the selection of the enzymes for his/her experiment. The user is guided through several decision steps in order to shortlist the best candidates, graphically presenting key information about the enzymes. Selenzyme is a key component of the automated pipeline at the SYNBIOCHEM where we are aiming to provide greener, more sustainable routes for microbial production of fine chemicals.

Carbonell, P., Wong, J., Swainston, N., Takano, E., Turner, N. J., Scrutton, N. S., Kell, D.B., Breitling, R. Faulon, J.L. (2018). Selenzyme: Enzyme selection tool for pathway design. Bioinformatics, bty065. http://doi.org/10.1093/bioinformatics/bty065

RetroPath2.0 workflow for Retrosynthesis

Retrosynthesis is a long-standing problem first introduced by Chemistry Nobel Laureate J.E. Corey in the late 1960s.  Taking advantage of recent progresses made in machine learning the problem has been revisited. In two papers SYNBIOCHEM teamed up with MICALIS Institute at INRA (France) to explore the use of retrosynthesis in biology. Both articles are making use of RetroPath2.0 a versatile, open source, easy to use and modifiable scientific workflow. In the first paper [1] the tool is used to perform retrosynthesis in the context of metabolic and biosensor engineering and is validated using actual examples of successful experimental implementations. In the second paper [2] the tool is used to perform inverse molecular design and search bioactive molecules in the chemical space as well as to create extended metabolic maps that can be used to annotate mass spectrometry data coming from a metabolomics pipeline. Because RetroPath2.0 is freely available on the workflow repository platform myExperiment.org [3] and is solely based on open source tools it is anticipated that community contributions will likely expand further its features.

[1] Delépine B, Duigou T, Carbonell P, Faulon JL. RetroPath2.0: A retrosynthesis workflow for metabolic engineers. Metabolic Engineering 2017, https://doi.org/10.1016/j.ymben.2017.12.002

[2] Koch M, Duigou T, Carbonell P, Faulon JL. Molecular structures enumeration and virtual screening in the chemical space with RetroPath2.0. J. Cheminformatics 2017, in press https://doi.org/10.1101/158147

[3] RetroPath2.0 download page: https://www.myexperiment.org/workflows/4987.html

New Synthetic Biology Collaboration with BGI

Patrick (Yizhi) Cai (Prof. Synthetic Genomics) at the University of Manchester and the President of  the Life Sciences Research Institute, Xun Xu from BGI signed a memorandum of Understanding to promote strategic collaboration. The Beijing Genomics Institute (BGI) and University of Manchester have launched a five-year collaboration focused on Synthetic Biology and metabolic engineering of natural products. The collaboration builds on Prof Patrick Cai’s synthetic genomics research and his involvement in the Synthetic Yeast Genome Project (sc 2.0) which aims to redesign and synthesize a 12-Mb designer yeast genome de novo.

Coupling droplet microfluidics with Mass Spectrometry

Emily Kempa: With the number of variants in a combinatorial library regularly exceeding 105 entities, SYNBIOCHEM Manchester are looking for new high throughput technologies for screening these variations for useful mutations to take forward into the next round of development. Although the Sphere Fluidics droplet platform and the FACS system are regularly utilised in the department for this use, their inherent downfall is the reliance upon the target compound to be fluorescent. To overcome this, a label-free approach in which the Sphere Fluidics droplet platform will be modified and coupled to a mass spectrometer has been devised.

This project has begun by coupling a droplet microfluidic chip with mass spectrometry through the insertion of a stainless steel emitter into a chip, and integrating this chip with the Agilent 6560 IM Q-TOF Mass Spectrometer. Droplets (~2 nL in size) are generated in the chip at a rate of 5 Hz and are introduced into the MS almost immediately with each droplet being detected as a distinct peak in the total ion chromatogram. Each peak in this chromatogram allows us to obtain a mass spectrum for each droplet, something that will be come of up-most importance when filling droplets with individual variants from the library or even monitoring reaction progress.

Discover our Research on YouTube

Watch our new University of Manchester Chemistry videos on YouTube! HERE

Demystifying the science behind our research papers, these videos provide a real world view of how important these discoveries are for day to day life.

These include: Finding new routes to replacement molecules for oil using yeast; Developing new routes to cheaper, better drugs by pairing man-made synthesis with natural enzymes; and harnessing the power of enzymes to make useful molecules for pharmaceuticals, polymers and plastics using alcohol.