High Throughput Robotics
HIGH THROUGHPUT ROBOTICS
SYNBIOCHEM has major robotics platforms for automated directed evolution and synthetic biology workflows.
Hamilton Star robotic platforms allow full flexibility and a number of on deck configurations with integrated incubators, PCR machines and plate readers. These have been programmed with SOPs to allow diverse experimental functionality including: sterile media dispensing and barcoded sample management; cloning; colony picking, cell pellet resuspension and lysis steps; fully automated pathway assembly protocols (e.g. LCR); automated PCR, DNA clean-up and manipulations; target product/protein extraction and purification protocols and a range of high-throughput assays (10*96) and analysis.
Tecan Freedom EVO 200
Tecan Freedom EVO 200 platform for generation of metabolite-sensitive aptamers through SELEX
ECHO650 acoustic liquid handling for high precision, non-contact, high speed, low volume (2.5nl) droplet transfer of diverse fluids from 384 and 1536-well microplates to destination plates or slides supporting a range of applications in drug discovery and basic research.
Fully Integrated Thermo System
A fully integrated Thermo system has now been installed on the 1st floor of MIB comprising: acoustic and pipette based liquid handling robots; plate handling robotic delivery arm; barcode readers; plate sealers/de-sealers; microplate centrifuge; plate reader; automated thermal cyclers (PCR machines) and incubators. Fully automated protocols for: generation of DNA libraries; culturing, transformation, incubation and growth storage of bacterial colonies; strain transformations; high throughput screening assays and product extraction.
Dr Mark Dunstan – Mark.Dunstan@manchester.ac.uk